Bioinformatics for Comparative Proteomics | Cathy H. Wu | Springer
Toggle navigation. Until recently, Cryptosporidium was thought to be a single species genus.
Molecular studies now show that there are at least 10 valid species of this parasite. Among them, two morphologically identical species, C. Post-genomic analyses is therefore necessary to explore further the genetic variations among them.
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During this study, a comparative proteomic approach was applied to analyze the differential expression of sporozoite proteins in both C. Using 2-DE gels with different pH ranges and 4- 7 and automated three dimensional 3D image analyses, a total of 20 protein spots were shown to be differentially expressed between the two species. Mass spectrometry analyses of these spots identified one hypothetical protein, however, identification of the remaining spots was unsuccessful.
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