Manual Proteins and Tumour Markers May 1995: Part 3

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Biochem Pharmacol. Coussens LM, Werb Z. Inflammation and cancer. Are the symptoms of cancer and cancer treatment due to a shared biologic mechanism? A cytokine-immunologic model of cancer symptoms. Relationship of serum levels of interleukin-6, soluble interleukin-6 receptor and tumor necrosis factor receptors to the acute-phase protein response in advanced pancreatic cancer.

Clin Sci Lond. View Article Google Scholar The role of C-reactive protein as a prognostic indicator in advanced cancer. Curr Oncol Rep. A systematic review of prognostic tools for estimating survival time in palliative care. J Palliat Care. Research electronic data capture REDCap —a metadata-driven methodology and workflow process for providing translational research informatics support. J Biomed Inform. International journal of surgery London, England.

Interleukin-6 and its surrogate C-reactive protein are useful serum markers for monitoring metastasized malignant melanoma. J Exp Clin Cancer Res. Diagnosing melanoma patients entering American Joint Committee on Cancer stage IV, C-reactive protein in serum is superior to lactate dehydrogenase. Br J Cancer. Comparative evaluation of serum C-reactive protein CRP levels in the different histological subtypes of esophageal cancer squamous cell carcinoma and adenocarcinoma of esophagus.

J Clin Lab Anal. Clinical utility of the pretreatment glasgow prognostic score in patients with advanced inoperable non-small cell lung cancer. J Thorac Oncol. Acute-phase protein response, survival and tumour recurrence in patients with colorectal cancer. Br J Surg. Association of C-reactive protein levels and long-term survival after neoadjuvant therapy and esophagectomy for esophageal cancer. J Gastrointest Surg. Serum acute phase reactants and prognosis in renal cell carcinoma.

Prognostic factors for renal cell carcinoma: a multivariate analysis of cases. Int J Urol. Multivariate analysis of prognostic determinants after surgery for renal cell carcinoma at Himeji National Hospital. Hinyokika Kiyo. Predictive values of acute phase reactants, basic fetoprotein, and immunosuppressive acidic protein for staging and survival in renal cell carcinoma. Clinical impact of the presence of the worst nucleolar grade in renal cell carcinoma specimens. Jpn J Clin Oncol. Prognostic factors of renal cell carcinoma with extension into inferior vena cava. Association of serum amyloid A protein and peptide fragments with prognosis in renal cancer.

C-reactive protein is a strong predictor for anaemia in renal cell carcinoma: role of IL-6 in overall survival. BJU Int. Intratumor C-reactive protein as a biomarker of prognosis in localized renal cell carcinoma. J Urol. Preoperative C-reactive protein level adjusted for comorbidities and lifestyle factors predicts overall mortality in localized renal cell carcinoma. Mol Diagn Ther. Interleukin-2, interferon-alpha and medroxyprogesterone acetate in metastatic renal cell carcinoma.

Anticancer Res. Radiation pneumonitis in lung cancer patients: a retrospective study of risk factors and the long-term prognosis. Interleukin 2 therapy in cancer: identification of responders.

Proteins and Tumour Markers May 1995

Serum level of interleukin 6 as a prognosis factor in metastatic renal cell carcinoma. Cancer Res. Serum C-reactive protein level and the impact of cytoreductive surgery in patients with metastatic renal cell carcinoma. Metastatic renal carcinoma comprehensive prognostic system. The systemic inflammatory response, performance status and survival in patients undergoing alpha-interferon treatment for advanced renal cancer.

C-reactive protein: a biomarker of survival in patients with metastatic renal cell carcinoma treated with subcutaneous interleukin-2 based immunotherapy. Prognostic factors for survival in patients with advanced renal cell carcinoma undergoing nonmyeloablative allogeneic stem cell transplantation. Prognostic factors in metastatic renal cell carcinoma: metastasectomy as independent prognostic variable. Improving the accuracy of pre-operative survival prediction in renal cell carcinoma with C-reactive protein. Postoperative better than preoperative C-reactive protein at predicting outcome after potentially curative nephrectomy for renal cell carcinoma.

Prospective study of the relationship between the systemic inflammatory response, prognostic scoring systems and relapse-free and cancer-specific survival in patients undergoing potentially curative resection for renal cancer. The relationship between the local and systemic inflammatory responses and survival in patients undergoing resection for localized renal cancer.

Impact of thrombocytosis and C-reactive protein elevation on the prognosis for patients with renal cell carcinoma.

DIETARY PROTEIN: HEALTH AND WELL BEING

Absolute preoperative C-reactive protein predicts metastasis and mortality in the first year following potentially curative nephrectomy for clear cell renal cell carcinoma. Prognostic significance of preoperative C-reactive protein elevation and thrombocytosis in patients with non-metastatic renal cell carcinoma. Korean J Urol. Impact of postoperative C-reactive protein level on recurrence and prognosis in patients with N0M0 clear cell renal cell carcinoma. Characteristics of aggressive variants in T1a renal cell carcinoma. J Cancer Res Clin Oncol.

The role of acute-phase reactant proteins, carcinoembryonic antigen and CA 19—9 as a marker in the preoperative staging of colorectal cancer: a prospective clinical study. Int Surg. Clinical significance of preoperative serum vascular endothelial growth factor, interleukin-6, and C-reactive protein level in colorectal cancer. BMC Cancer. Host markers and prognosis in recurrent rectal carcinomas treated with radiotherapy.

Acta Oncol. Significance of inflammatory cytokines in the progression of colorectal cancer. Preoperative but not postoperative systemic inflammatory response correlates with survival in colorectal cancer. Tumor size is associated with the systemic inflammatory response but not survival in patients with primary operable colorectal cancer.

J Gastroenterol Hepatol. C-reactive protein concentration is associated with prognosis in patients suffering from peritoneal carcinomatosis of colorectal origin. Int J Colorectal Dis. Evaluation of predictive markers for patients with advanced colorectal cancer. Prognostic value of an inflammation-based score in patients undergoing pre-operative chemotherapy followed by surgery for esophageal cancer. Exp Ther Med. Clinical significance of tumor pathology for postoperative survival of patients undergoing surgery for stage IV colorectal cancer.

Serum C-reactive protein correlates with survival in colorectal cancer patients but is not an independent prognostic indicator. Eur J Gastroenterol Hepatol. Practical prognostic index for patients with metastatic pancreatic cancer treated with gemcitabine. Prognostic factors in japanese patients with advanced pancreatic cancer treated with single-agent gemcitabine as first-line therapy. Tumour Biol. The glasgow prognostic score as a predictor of survival in patients with potentially resectable pancreatic adenocarcinoma.

Ann Surg Oncol. Clinical significance of pretreatment C-reactive protein in patients with advanced nonsquamous, non-small cell lung cancer who received gefitinib. Postoperative serum C-reactive protein levels in non-small cell lung cancer patients. Ann Thorac Cardiovasc Surg. The Glasgow Prognostic Score GPS predicts toxicity and efficacy in platinum-based treated patients with metastatic lung cancer.

Lung Cancer.

Mini-review: The T Cell Marker, CD3 Antigen and Antibodies

Impact of an inflammation-based prognostic system on patients undergoing surgery for hepatocellular carcinoma: a retrospective study of Japanese patients. Am J Surg. A novel and validated prognostic index in hepatocellular carcinoma: the inflammation based index IBI. J Hepatol. Pretreatment serum C-reactive protein level predicts poor prognosis in patients with hepatocellular carcinoma. Med Oncol. Predictors of clinical response to interleukin-2—based immunotherapy in melanoma patients: a French multiinstitutional study.

J Clin Oncol. Elevated pre-treatment levels of plasma C-reactive protein are associated with poor prognosis after breast cancer: a cohort study. Breast Cancer Res. Ravishankaran P, Karunanithi R. Clinical significance of preoperative serum interleukin-6 and C-reactive protein level in breast cancer patients. World J Surg Oncol. Morizane S. Serum C-reactive protein level is significant prognostic indicator in patients with advanced urothelial cancer treated with Gemcitabine-Cisplatin or Carboplatin-preliminary results.

Central European Journal of Urology. C-reactive protein: A biomarker of survival in patients with localized upper tract urothelial carcinoma treated with radical nephroureterectomy. Urol Oncol. C-reactive protein level predicts prognosis in patients with muscle-invasive bladder cancer treated with chemoradiotherapy. C-reactive protein as a prognostic marker for men with androgen-independent prostate cancer: results from the ASCENT trial.

Prognostic impact of C-reactive protein for determining overall survival of patients with castration-resistant prostate cancer treated with docetaxel. Systemic inflammation and survival of patients with prostate cancer: evidence from the Glasgow Inflammation Outcome Study. Prostate Cancer Prostatic Dis. C-reactive protein is a prognostic parameter in patients with cervical cancer. Gynecol Oncol. Serum C-reactive protein as independent prognostic variable in patients with ovarian cancer.

Clin Cancer Res. Clinical significance of pretreatment serum C-reactive protein level in soft tissue sarcoma. Serum C-reactive protein as a prognostic factor in patients with epithelial ovarian cancer. Prognostic value of the doubling time of serum C-reactive protein and alkaline phosphatase levels in primary bone and soft tissue tumors.

Jpn J Cancer Res. Baseline C-reactive protein is associated with incident cancer and survival in patients with cancer. Thrombosis risk and survival in cancer patients with elevated C-reactive protein. J Thromb Haemost. Associations among hypogonadism, C-reactive protein, symptom burden, and survival in male cancer patients with cachexia: a preliminary report. J Pain Symptom Manage. Prognostic parameters determining survival in pancreatic carcinoma and, in particular, after palliative treatment.

Dig Dis. Mini Nutritional Assessment MNA and biochemical markers of cachexia in metastatic lung cancer patients: interrelations and associations with prognosis. Prognostic significance of a systemic inflammatory response in patients receiving first-line palliative chemotherapy for recurred or metastatic gastric cancer. Prognostic relevance of oncological serum biomarkers in liver cancer patients undergoing transarterial chemoembolization therapy. Utility of inflammation-based prognostic scoring in patients given systemic chemotherapy first-line for advanced inoperable bladder cancer.

Inflammatory markers in blood and serum tumor markers predict survival in patients with epithelial appendiceal neoplasms undergoing surgical cytoreduction and intraperitoneal chemotherapy. Ann Surg. Systemic inflammation and prediction of chemotherapy outcomes in patients receiving docetaxel for advanced cancer. Support Care Cancer. Preoperative serum C-reactive protein and gastric cancer; clinical-pathological correlation and prognostic significance. Chang Gung Med J. Clinical implications of serum C-reactive protein levels in malignant fibrous histiocytoma.

Int J Cancer. Prognostic significance of serum CRP levels and lymphoid cell infiltrates in human breast cancer. Tumour markers as prognostic aids in prostatic carcinoma. Br J Urol. Ekman P, Lewenhaupt A. Serum tumour markers in human prostatic carcinoma.


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The value of a marker panel for prognostic information. The role of serum tumour markers to aid the selection of lung cancer patients for surgery and the assessment of prognosis. Eur J Cancer Clin Oncol. Clinical prognostic scoring system to aid decision-making in gastro-oesophageal cancer. Clinical significances of preoperative serum interleukin-6 and C-reactive protein level in operable gastric cancer. Clin Exp Med. Acute phase proteins in patients with large and locally advanced breast cancer treated with neo-adjuvant chemotherapy: response and survival.

Int J Oncol. Serum levels of leptin and proinflammatory cytokines in advanced-stage non-small cell lung cancer. Ann Oncol. Glare P. Clinical predictors of survival in advanced cancer. J Support Oncol. Tumor marker utility grading system: a framework to evaluate clinical utility of tumor markers. Tumor markers as clinical cancer tests—are we there yet? Semin Oncol. Nat Clin Pract Urol. High-sensitivity C-reactive protein levels and cancer mortality.

Cancer Epidemiol Biomarkers Prev. Presence of tumoural C-reactive protein correlates with progressive prostate cancer. Tumoral CRP expression in thoracic esophageal squamous cell cancers is associated with poor outcomes. Surg Today. Impact of C-reactive protein kinetics on survival of patients with metastatic renal cell carcinoma.

Eur Urol. Prognostic impact of postoperative C-reactive protein level in patients with metastatic renal cell carcinoma undergoing cytoreductive nephrectomy. Association of C-reactive protein gene polymorphisms and colorectal cancer. Genetic variation in C-reactive protein in relation to colon and rectal cancer risk and survival. Assessment of nutritional status and prognosis in advanced cancer: interleukin-6, C-reactive protein, and the prognostic and inflammatory nutritional index.

Comparison of an inflammation-based prognostic score GPS with performance status ECOG-ps in patients receiving palliative chemotherapy for gastroesophageal cancer. Comparison of the prognostic value of tumour and patient related factors in patients undergoing potentially curative resection of gastric cancer. BMC Public Health. Evaluation of an inflammation-based prognostic score GPS in patients undergoing resection for colon and rectal cancer.

What does minor elevation of C-reactive protein signify? Am J Med. Levels of some acute-phase proteins in the serum of patients with cancer during radiotherapy. Biol Pharm Bull. Bland M, editor. An Introduction to Medical Statistics. NY: Oxford University Press; A systematic review of the association between circulating concentrations of C reactive protein and cancer.

J Epidemiol Community Health. Balkwill F, Mantovani A. Inflammation and cancer: back to Virchow? Developing a rational tumor vaccine therapy for renal cell carcinoma: immune yin and yang. Fantini MC, Pallone F.

Tumor Markers in Cancer diagnosis and Monitoring

Cytokines: from gut inflammation to colorectal cancer. Curr Drug Targets. Plasma fibrinogen and serum C-reactive protein are associated with non-small cell lung cancer. Definition of cancer cachexia: effect of weight loss, reduced food intake, and systemic inflammation on functional status and prognosis. Am J Clin Nutr. C-reactive protein and all-cause mortality in a large hospital-based cohort.

Clin Chem.

What is it used for?

Prognostic markers in cancer: the evolution of evidence from single studies to meta-analysis, and beyond. Significant prognostic factors for 5-year survival after curative resection of renal cell carcinoma. Prognostic impact of in vivo soluble cell adhesion molecules in metastatic renal cell carcinoma. The relationship between the preoperative systemic inflammatory response and cancer-specific survival in patients undergoing potentially curative resection for renal clear cell cancer. An approach to estimating prognosis using fractional polynomials in metastatic renal carcinoma.

Increased preoperative serum C-reactive protein level predicts a poor prognosis in patients with localized renal cell carcinoma. Evaluation of an inflammation-based prognostic score in patients with metastatic renal cancer. C-reactive protein is an informative predictor of renal cell carcinoma-specific mortality: a European study of patients.

Basal cytokines profile in metastatic renal cell carcinoma patients treated with subcutaneous ILbased therapy compared with that of healthy donors. J Transl Med. How do symptoms have an impact on the prognosis of renal cell carcinoma? Development and external validation of a new outcome prediction model for patients with clear cell renal cell carcinoma treated with nephrectomy based on preoperative serum C-reactive protein and TNM classification: the TNM-C score.

Prognostic factors for renal cell carcinoma with bone metastasis: who are the long-term survivors? Is Memorial Sloan-Kettering Cancer Center risk classification appropriate for Japanese patients with metastatic renal cell carcinoma in the cytokine era? Soga N. The impact of preoperative serum albumin level and postoperative C-reactive protein nadir on the survival of patients with non-metastatic renal cell carcinoma with vessel thrombus after nephrectomy. Curr Urol. Clinical utility of the Glasgow Prognostic Score in patients undergoing curative nephrectomy for renal clear cell cancer: basis of new prognostic scoring systems.

Prospective study of the role of inflammation in renal cancer. Urol Int. C-reactive protein as a prognostic marker for advanced renal cell carcinoma treated with sunitinib. Prognostic impact of pretreatment C-reactive protein for patients with metastatic renal cell carcinoma treated with tyrosine kinase inhibitors. Int J Clin Oncol.

Impact of hyponatremia on survival of patients with metastatic renal cell carcinoma treated with molecular targeted therapy. Inflammatory prognostic markers in clear cell renal cell carcinoma—preoperative C-reactive protein does not improve predictive accuracy. Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma. Validation of CRP as prognostic marker for renal cell carcinoma in a large series of patients. Significance of preoperative elevation of serum C-reactive protein as an indicator for prognosis in colorectal cancer.

Preoperative plasma plasminogen activator inhibitor type-1 and serum C-reactive protein levels in patients with colorectal cancer. Systemic inflammatory response predicts survival following curative resection of colorectal cancer. The effect of deprivation and the systemic inflammatory response on outcome following curative resection for colorectal cancer. C-reactive protein as a prognostic variable that reflects uncontrolled up-regulation of the ILIL-6 network system in colorectal carcinoma.

Dig Dis Sci. Evaluation of a cumulative prognostic score based on the systemic inflammatory response in patients undergoing potentially curative surgery for colorectal cancer. Portal and systemic serum growth factor and acute-phase response after laparotomy or partial hepatectomy in patients with colorectal liver metastases: a prognostic role for C-reactive protein and hepatocyte growth factor. Scand J Gastroenterol.

World J Gastroenterol. The relationship between tumour T-lymphocyte infiltration, the systemic inflammatory response and survival in patients undergoing curative resection for colorectal cancer. Evaluation of nutritional and inflammatory status of advanced colorectal cancer patients and its correlation with survival. Nutr Cancer.

Proteins and Tumour Markers May Part 3 by Hugh Young - tyruvyvizo.cf

The presence of a systemic inflammatory response predicts poorer survival in patients receiving adjuvant 5-FU chemotherapy following potentially curative resection for colorectal cancer. The relationship between tumour proliferative activity, the systemic inflammatory response and survival in patients undergoing curative resection for colorectal cancer. Colorectal Dis. Comparison of the prognostic value of selected markers of the systemic inflammatory response in patients with colorectal cancer. Preoperative prognostic score for predicting survival after hepatic resection for colorectal liver metastases.

Inflammation-based prognostic score is a novel predictor of postoperative outcome in patients with colorectal cancer. Is C-reactive protein a prognostic factor of colorectal cancer? Dis Colon Rectum. Preoperative C-reactive protein as a prognostic and therapeutic marker for colorectal cancer. J Surg Oncol. Preoperative elevation of serum C-reactive protein as an independent prognostic indicator of colorectal carcinoma.

Clin Chem Lab Med. Systemic inflammatory response predicts prognosis in patients with advanced-stage colorectal cancer. Clin Colorectal Cancer. Relationship between emergency presentation, systemic inflammatory response, and cancer-specific survival in patients undergoing potentially curative surgery for colon cancer. Systemic inflammatory response predicts postoperative outcome in patients with liver metastases from colorectal cancer. The relationship between the local and systemic inflammatory responses and survival in patients undergoing curative surgery for colon and rectal cancers.

Influence of inflammation-based prognostic score on mortality of patients undergoing chemotherapy for far advanced or recurrent unresectable colorectal cancer. Predictors of survival in stage IV metastatic colorectal cancer. Elevated C-reactive protein and hypoalbuminemia measured before resection of colorectal liver metastases predict postoperative survival. Dig Surg. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.

The relationship between patient physiology, the systemic inflammatory response and survival in patients undergoing curative resection of colorectal cancer. Evaluation of an inflammation-based prognostic score for the identification of patients requiring postoperative adjuvant chemotherapy for stage II colorectal cancer. High levels of acute phase proteins and soluble 70 kDa heat shock proteins are independent and additive risk factors for mortality in colorectal cancer. Cell Stress Chaperones. Relationship between preoperative comorbidity, systemic inflammatory response, and survival in patients undergoing curative resection for colorectal cancer.

Preoperative thrombocytosis is associated with survival after surgery for colorectal cancer. The Glasgow prognostic score is valuable for colorectal cancer with both synchronous and metachronous unresectable liver metastases. Oncol Lett. Glasgow prognostic score as a prognostic factor in patients undergoing curative surgery for colorectal cancer. Significant host- and tumor-related factors for predicting prognosis in patients with esophageal carcinoma.

Elevation of preoperative serum C-reactive protein level is related to poor prognosis in esophageal squamous cell carcinoma. Guillem P, Triboulet JP. Elevated serum levels of C-reactive protein are indicative of a poor prognosis in patients with esophageal cancer. Dis Esophagus. Significance of preoperative C-reactive protein as a parameter of the perioperative course and long-term prognosis in squamous cell carcinoma and adenocarcinoma of the oesophagus. An elevated C-reactive protein concentration, prior to surgery, predicts poor cancer-specific survival in patients undergoing resection for gastro-oesophageal cancer.

Inflammation-based prognostic score, prior to neoadjuvant chemoradiotherapy, predicts postoperative outcome in patients with esophageal squamous cell carcinoma. Higher serum C-reactive protein concentration and hypoalbuminemia are poor prognostic indicators in patients with esophageal cancer undergoing radiotherapy.

Radiother Oncol. Inflammation-based prognostic score and number of lymph node metastases are independent prognostic factors in esophageal squamous cell carcinoma. Elevated serum CRP levels after induction chemoradiotherapy reflect poor treatment response in association with IL-6 in serum and local tumor site in patients with advanced esophageal cancer.

Glasgow Prognostic Score is a predictor of perioperative and long-term outcome in patients with only surgically treated esophageal cancer. Comparison of the prognostic value of tumour- and patient-related factors in patients undergoing potentially curative resection of oesophageal cancer. World J Surg. The relationship between tumour necrosis, tumour proliferation, local and systemic inflammation, microvessel density and survival in patients undergoing potentially curative resection of oesophageal adenocarcinoma.

Elevated tumour interleukin-1beta is associated with systemic inflammation: A marker of reduced survival in gastro-oesophageal cancer. Plasma protein profiles and prognosis in gastric cancer. Comparison of pre-treatment clinical prognostic factors in patients with gastro-oesophageal cancer and proposal of a new staging system. Crumley ABC. Interrelationship between tumor proliferation activity, leucocyte and macrophage infiltration, systemic inflammatory response and survival in patients selected for potentially curative resection. Citations 0.

Red Loading Buffer Pack Reviews 4. BSA Reviews 4. Nonfat Dry Milk Reviews 4. Nitrocellulose Sandwiches Reviews 4. Citations Dilute to 1X with dH 2 O. Nonfat Dry Milk : Biotinylated Protein Ladder Detection Pack : Blotting Membrane and Paper : This protocol has been optimized for nitrocellulose membranes. Pore size 0. Protein Blotting A general protocol for sample preparation. Treat cells by adding fresh media containing regulator for desired time.

Aspirate media from cultures; wash cells with 1X PBS; aspirate.

Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Microcentrifuge for 5 min. Electrotransfer to nitrocellulose membrane Membrane Blocking and Antibody Incubations NOTE : Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Wash three times for 5 min each with 15 ml of TBST. Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody at and anti-biotin, HRP-linked Antibody at — to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.

Proceed with detection Section D. Mix well. Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wet , wrap in plastic and expose to X-ray film. Species Reactivity: Human, Mouse, Monkey. Wang, Y. Hirata, H. Hoffman, L.